个人简介

李文涛，男，四川南充人。华中农业大学教授，硕士、博士生导师。四川农业大学学士、华中农业大学农学博士、荷兰乌特勒支大学哲学博士（兽医病毒方向）。全国动物病原微生物菌（毒）种保藏管理专家委员会委员，荷兰皇家微生物协会会员，Animal Diseases杂志的Science Editor，湖北洪山实验室固定研究人员，Emerging Microbes & Infections等期刊的审稿人。主要从事冠状病毒相关研究，包括病毒的入侵机制、新病毒的分离、新型疫苗和治疗性单克隆抗体的研发等，取得了一系列具有国际影响力的创新性研究成果：a) 报道了全球首株针对SARS-CoV-2的治疗性单克隆抗体，并解析了其与S蛋白的复合物结构，该抗体已经成功转化，正在美国进行I期临床实验；b) 首次报道了高致病性PEDV变异毒株在中国的流行及其序列特征；c) 鉴定了PDCoV的受体-氨基肽酶N（APN），并揭示该病毒的来源以及跨物种感染人的潜力；d) 鉴定了MERS-CoV的辅助受体，阐明了其感染宿主的种属特异性和组织嗜性的分子基础。近年在PNAS (2篇)、Nat. Commun. (1篇)、Sci. Adv.(1篇)、 Emerging Infect. Dis. (2篇) 和J. Virol. (2篇)等国际重要刊物上发表冠状病毒相关SCI论文40余篇，总引用频次超过4300次，其中两篇论文被选为ESI高被引论文。本实验室注重培养学生学以致用，并与荷兰乌特勒支大学兽医学院建立了长期的合作关系，支持学生到国外开展合作研究，欢迎优秀的同学和博士后加盟！

教育经历

2004.09 - 2008.06，四川农业大学，生物工程专业，工学学士
2008.09 - 2014.12，华中农业大学，预防兽医学专业，农学博士
2013.05 – 2014.12，荷兰乌特勒支大学兽医学院，联合培养博士研究
2015.01 - 2018.02，荷兰乌特勒支大学兽医学院，兽医病毒学专业，哲学博士

工作经历

2015.07–2020.12，荷兰乌特勒支大学兽医学院，博士后
2020.12- 至今，华中农业大学教授、博士生导师

研究领域

主要从新病毒的发现、分子流行病学、分离培养、致病机制、新型疫苗、抗病毒药物和治疗性单克隆抗体的研发等方向开展研究工作。
非洲猪瘟病毒和猪腹泻相关冠状病毒入侵机制、跨种传播机制、抗病毒药物和新型疫苗的研究。

科研成果

B论文类
1.Fedry J#, Hurdiss DL#, Wang C#, Li W#, Obal G, Drulyte I, Du W, Howes SC, van Kuppeveld FJM, Förster F*, Bosch BJ*. Structural insights into the cross-neutralization of SARS-CoV and SARS-CoV-2 by the human monoclonal antibody 47D11. Sci Adv. 2021 May 6:eabf5632. doi: 10.1126/sciadv.abf5632. (#Co-first author)
2.Wang, C.#, Li, W.#, Drabek, D#., Okba, N., van Haperen, R., Osterhaus, A., van Kuppeveld, F., Haagmans, B. L., Grosveld, F., & Bosch, B. J.* (2020). A human monoclonal antibody blocking SARS-CoV-2 infection. Nature Communications, 11(1), 1-6. (#Co-first author，ESI高被引论文)
3.Okba, N. M.#, Müller, M. A.#, Li, W.#, Wang, C., GeurtsvanKessel, C. H., Corman, V. M., Lamers, M. M., Sikkema, R. S., de Bruin, E., Chandler, F. D., Yazdanpanah, Y., Le Hingrat, Q., Descamps, D., Houhou-Fidouh, N., Reusken, C., Bosch, B. J., Drosten, C., Koopmans, M., & Haagmans, B. L*. (2020). Severe acute respiratory syndrome coronavirus 2-specific antibody responses in coronavirus disease 2019 patients. Emerging Infectious Diseases, 26(7). (#Co-first author，ESI高被引论文和热点论文)
4.Li. W.#, R. J. G. Hulswit#, S. P. Kenney#, I. Widjaja, K. Jung, M. A. Alhamo, B. van Dieren, F. J. M. van Kuppeveld, L. J. Saif*, B. J. Bosch*, (2018). Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibility. PNAS, 115 (22), E5135-E5143.
5.Li. W.#, R. J. G. Hulswit#, I. Widjaja#, V. S. Raj, R. McBride, W. Peng, W. Widagdo, M. A. Tortorici, B. van Dieren, Y. Lang, J. W. M. van Lent, J. C. Paulson, C. A. M. de Haan, R. J. de Groot, F. J. M. van Kuppeveld, B. L. Haagmans and B. J. Bosch* (2017). Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein. PNAS, 114 (40), E8508-E8517.
6.Li, C.#, Li, W.#, de Esesarte, E.L., Guo, H., van den Elzen, P., Aarts, E., van den Born, E., Rottier, P.J., Bosch, B. J.* (2017). Cell Attachment Domains of the Porcine Epidemic Diarrhea Virus Spike Protein Are Key Targets of Neutralizing Antibodies. Journal of Virology, 91(12): e00273-17. (#Co-first author)
7.Li, W., Luo, R., He, Q., van Kuppeveld, F. J., Rottier, P. J., & Bosch, B. J. *(2017). Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry. Virus Research, 235: 6-13.
8.Li, W., van Kuppeveld, F. J., He, Q., Rottier, P. J., & Bosch, B. J.* (2016). Cellular entry of the porcine epidemic diarrhea virus. Virus Research, 226, 117-127.
9.Li, W., Wicht, O., van Kuppeveld, F. J., He, Q., Rottier, P. J., & Bosch, B. J.* (2015). A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion. Journal of virology, 89(15), 8077-8081.
10.Li, W., Liu, S., Wang, Y., Deng, F., Yan, W., Yang, K., Chen, H., He, Q.*, Charreyre, C., Audonnet, J.-C. (2013). Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2. BMC genomics, 14(1), 353.
11.Li W., Li H., Liu Y., Pan Y., Deng F., Song Y., Tang X., He Q.* (2012). New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis. 18(8): 1350-1353.
12.Yang, K.#, Li, W.#, Niu, H., Yan, W., Liu, X., Wang, Y., Cheng, S., Ku, X., He, Q.* (2012). Efficacy of single dose of an inactivated porcine circovirus type 2 (PCV2) whole-virus vaccine with oil adjuvant in piglets. Acta Veterinaria Scandinavica, 54(1), 67. (#Co-first author)
13.Ni, Y., Rosier, B. J., van Aalen, E. A., Hanckmann, E. T., Biewenga, L., Pistikou, A. M. M., Timmermans, B., Vu, C., Roos, S., Arts R., Li, W., de Greef, TFA., van Borren, M., van Kuppenveld, F.J.M, Bosch, BJ & Maarten Merkx& Merkx, M. (2021). A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays. Nature communications, 12(1), 1-12.
14.Wang C., van Haperen R., Gutiérrez-Álvarez J, Li, W., Okba, N. M. A., Albulescu, I., Widjaja, I., van Dieren, B., Fernandez-Delgado, R., Sola, I., Hurdiss, D. L., Daramola, O., Grosveld, F., van Kuppeveld, F. J. M., Haagmans, B. L., Enjuanes, L., Drabek, D., Bosch, B. J. (2021) A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies. Nature Communications, 2021, 12(1): 1-15.
15.Zhao, S., Schuurman, N., Li, W., Wang, C., Smit, L.A., Broens, E., Wagenaar, J., van Kuppeveld, F.J., Bosch, B.J., Egberink, H., (2021). Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands. Emerging Infectious Disease, 2021, 27(5).
16.Okba, N. M., Widjaja, I., Li, W., GeurtsvanKessel, C. H., Farag, E., Al-Hajri, M., Park, W. B., Oh, M. D., Reusken, C., Koopmans, M., Bosch, B. J., & Haagmans, B. L. (2020). Serologic detection of middle east respiratory syndrome coronavirus functional antibodies. Emerging infectious diseases, 26(5), 1024.
17.van Tol S, Mögling R, Li W, Godeke, G. J., Swart, A., Bergmans, B., Brandenburg, A., Kremer, K., Murk, J. L., van Beek, J., Wintermans, B., Reimerink, J., Bosch, B. J., & Reusken, C. (2020). Accurate Serology for SARS-CoV-2 and common Human Coronaviruses using a Multiplex Approach. Emerging Microbes & Infections: 1-24.
18.Vogelzang EH, Loeff FC, Derksen NIL, Kruithof S, Ooijevaar-de Heer P, van Mierlo G, Linty F, Mok JY, van Esch W, de Bruin S, Vlaar APJ; Amsterdam University Medical Center COVID-19 Biobank Study Group,, Seppen B, Leeuw M, van Oudheusden AJG, Buiting AGM, Jim KK, Vrielink H, Swaneveld F, Vidarsson G, van der Schoot CE, Wever PC, Li W, van Kuppeveld F, Murk JL, Bosch BJ, Wolbink GJ, Rispens T. (2020) Development of a SARS-CoV-2 Total Antibody Assay and the Dynamics of Antibody Response over Time in Hospitalized and Nonhospitalized Patients with COVID-19. J Immunol. 205(12):3491-3499. doi: 10.4049/jimmunol.2000767.
19.Saletti G, Gerlach T, Jansen JM, Molle A, Elbahesh H, Ludlow M, Li W, Bosch BJ, Osterhaus ADME, Rimmelzwaan GF. (2020) Older adults lack SARS CoV-2 cross-reactive T lymphocytes directed to human coronaviruses OC43 and NL63. Sci Rep. 10(1):21447. doi: 10.1038/s41598-020-78506-9.
20.Hulswit, R. J.#, Lang, Y.#, Bakkers, M. J.#, Li, W., Li, Z., Schouten, A., Ophorst, B., van Kuppeveld, F. J. M., Boons, G.J., Bosch, B.J., Huizinga, E. G., de Groot, R. J. (2019). Human coronaviruses OC43 and HKU1 bind to 9-O-acetylated sialic acids via a conserved receptor-binding site in spike protein domain A. PNAS, 116(7): 2681-2690.
21.Lang Y, Li W, Li Z, Koerhuis D, van den Burg ACS, Rozemuller E, Bosch BJ, van Kuppeveld FJM, Boons GJ, Huizinga EG, van der Schaar HM, de Groot RJ. (2020) Coronavirus hemagglutinin-esterase and spike proteins coevolve for functional balance and optimal virion avidity. PNAS. 117(41):25759-25770. doi: 10.1073/pnas.2006299117.
22.W. Widagdo#, N.M.A Okba#, W. Li, A. de Jong, R.Y.L. de Swart, L. Begeman, J.M.A van den Brand, B.J. Bosch, B.L. Haagmans. (2019). Species-specific co-localization of MERS-CoV attachment and entry receptors. Journal of Virology, JVI.00107-19; DOI: 10.1128/JVI.00107-19.
23.Park, Y. J., Walls, A. C., Wang, Z., Sauer, M. M., Li, W., Tortorici, M. A., DiMaio, F., Bosch, B.J., Veesler, D. (2019). Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors. Nature Structural & Molecular Biology, 26(12), 1151-1157.
24.Zhao S, Li W, Schuurman N, van Kuppeveld F, Bosch BJ, Egberink H (2019). Serological Screening for Coronavirus Infections in Cats. Viruses, 11(8): 743.
25.I. Widjaja, C. Wang, R. van Haperen, J. Gutiérrez-Álvarez, B. van Dieren, N.M.A. Okba, V. Stalin Raj, Li, W., R. Fernandez-Delgado, F. Grosveld, F.J. M. van Kuppeveld, B.L. Haagmans, L. Enjuanes, D. Drabek, B. J. Bosch (2019). Towards a solution to MERS: protective human monoclonal antibodies targeting different domains and functions of the MERS-coronavirus spike glycoprotein. Emerging microbes & infections 8.1: 516-530.
26.J.C.F.M. Dortmans, W. Li, P.J. van der Wolf, G.J. Buter, P.J.M. Franssena, G. van Schaik, M. Houben, B.J. Bosch. (2018). Porcine epidemic diarrhea virus (PEDV) introduction into a naive Dutch pig population in 2014. Veterinary Microbiology, 221: 13-18.
27.Liu S, Li W, Wang Y, Gu C, Liu X, Charreyre C, Fan S, He Q. (2017). Coinfection with Haemophilus parasuis serovar 4 increases the virulence of porcine circovirus type 2 in piglets. Virology journal. 14(1):227.
28.Xiong, X., Tortorici, M. A., Snijder, J., Yoshioka, C., Walls, A. C., Li, W., McGuire AT, Rey FA, Bosch BJ, Veesler, D. (2017). Glycan shield and fusion activation of a deltacoronavirus spike glycoprotein fine-tuned for enteric infections. Journal of Virology, 92(4): e01628-17.
29.Walls, A. C., Tortorici, M. A., Frenz, B., Snijder, J., Li, W., Rey, F.A., DiMaio, F., Bosch, B.J., Veesler, D. (2016). Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy. Nature structural & molecular biology, 23(10): 899-905.
30.Chen, J., Li, W., Jin, E., He, Q., Yan, W., Yang, H., Gong, S., Guo, Y., Fu, S., Chen, X (2016). The antiviral activity of arctigenin in traditional Chinese medicine on porcine circovirus type 2. Research in veterinary science, 106, 159-164.
31.Ye, S., Li, Z., Chen, F., Li, W., Guo, X., Hu, H., & He, Q. (2015). Porcine epidemic diarrhea virus ORF3 gene prolongs S-phase, facilitates formation of vesicles and promotes the proliferation of attenuated PEDV. Virus genes, 51(3), 385-392.
32.Wicht, O., Li, W., Willems, L., Meuleman, T.J., Wubbolts, R.W., van Kuppeveld, F.J., Rottier, P.J., Bosch, B.J. (2014). Proteolytic activation of the porcine epidemic diarrhea coronavirus spike fusion protein by trypsin in cell culture. Journal of Virology, 88(14), 7952-7961.
33.Shao, F., Lu, Z., Liu, C., Han, H., Chen, K., Li, W., He, Q., Peng, H., Chen, J. (2014). Hierarchical nanogaps within bioscaffold arrays as a high-performance SERS substrate for animal virus biosensing. ACS applied materials & interfaces, 6(9), 6281-6289.
34.Wang, C., Chen, F., Hu, H., Li, W., Wang, Y., Chen, P., Liu, Y., Ku, X., He, Q., Chen, H. (2014). Gene expression profiling of cecropin B-resistant Haemophilus parasuis. Journal of molecular microbiology and biotechnology, 24(2), 120-129.
35.Hu, H., Wang, C., Guo, X., Li, W., Wang, Y., & He, Q. (2013). Broad activity against porcine bacterial pathogens displayed by two insect antimicrobial peptides moricin and cecropin B. Molecules and cells, 35(2), 106-114.
36.Zhang, H., Liu, C., Cheng, S., Wang, X., Li, W., Charreyre, C., Audonnet, J.C., He, Q. (2013). Porcine CD74 is involved in the inflammatory response activated by nuclear factor kappa B during porcine circovirus type 2 (PCV-2) infection. Archives of virology, 158(11), 2285-2295.
37.Luo, Z., Li, W., Lu, D., Chen, K., He, Q., Han, H., & Zou, M. (2013). A SERS-based immunoassay for porcine circovirus type 2 using multi-branched gold nanoparticles. Microchimica Acta, 180(15-16), 1501-1507.
38.Chen, L. #, Wang, J. #, Li, W., & Han, H. (2012). Aqueous one-pot synthesis of bright and ultrasmall CdTe/CdS near-infrared-emitting quantum dots and their application for tumor targeting in vivo. Chemical Communications, 48(41), 4971-4973.
39.Cheng, S., Zhang, M., Li, W., Wang, Y., Liu, Y., & He, Q. (2012). Proteomic analysis of porcine alveolar macrophages infected with porcine circovirus type 2. Journal of proteomics, 75(11), 3258-3269.
40.Wang, Y., Liu, C., Fang, Y., Liu, X., Li, W., Liu, S., Liu, Y., Liu, Y., Charreyre, C., Audonnet, J.-C., Chen, P., & He, Q,. (2012). Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis. BMC genomics, 13(1), 68.
41.Liu, Y., Chen, P., Wang, Y., Li, W., Cheng, S., Wang, C., Zhang, A., He, Q. (2012). Transcriptional profiling of Haemophilus parasuis SH0165 response to tilmicosin. Microbial drug resistance, 18(6), 604-615.
42.Wang, Y., Fang, Y., Liu, Y., Chen, P., Li, W., Liu, S., Zou, H., He, Q., (2010). Development and evaluation of loop-mediated isothermal amplification for rapid detection of Haemophilus parasuis. FEMS microbiology letters, 313(1), 54-60.
43.Zhang, H., Li, W., Sheng, Z., Han, H., & He, Q. (2010). Ultrasensitive detection of porcine circovirus type 2 using gold (III) enhanced chemiluminescence immunoassay. Analyst, 135(7), 1680-1685.

科研项目

1.华中农业大学高层人才启动基金, 2021-2026, 主持
2.MANCO - Monoclonal Antibodies against 2019-New Coronavirus, funded by EU. 2020-2023. 参与
3.INATAC: Innovative Antibody Therapies Against COVID-19. 2020-2023. 参与
4.IMI-ZAPI grant (Zoonoses Anticipation and Preparedness Initiative (ZAPI) project, funded by EU and EFPIA partners; IMI Grant no.115760). 2015-2020. 参与
5.ESCMID Research Award ‘Propagation of enteric coronaviruses: No guts no glory’, 2015-2017. 参与
6.Research grant on vaccine development for porcine epidemic diarrhea virus. Funded by MSD-AH, 2015-2018. 参与
7.Research grant on vaccine development for porcine epidemic diarrhea virus. Funded by MSD-AH, 2013-2014. 参与
8.猪流行性腹泻病毒变异株感染性克隆构建及致病机制研究。国家自然科学基金面上项目(31272572/ C1805), 2013-2016. 参与

主要奖励

1.2019年，FEMS Meeting Attendance Grant；
2.2018年，国家留学基金委，优秀自费留学生；
3.2017年，FEMS Meeting Attendance Grant；
4.2017年，荷兰乌特勒支大学，Best Publication Prize；
5.2012 年，博士研究生国家奖学金；
6.2012年，大北农奖学金；
7.2012年，华中农业大学，研究生“学术希望之星”;
8.2012年，华中农业大学，“三好研究生标兵”
9.2010年，中国畜牧兽医学会优秀论文；