个人简介

苗义良，博士，教授，博士生导师，现工作于华中农业大学动科动医学院。动物繁殖与特经系主任，干细胞与再生生物学研究所所长，华中农业大学实验动物中心副主任。兼任中国动物学会细胞与分子显微技术学会常务委员、中国动物学会生殖生物学会委员、中国农业生物技术学会动物生物技术分会委员、《华中农业大学学报》副主编等。主要致力于1）动物早期胚胎发育和体细胞重编程的机制；2）胚胎干细胞的构建与自我更新机制研究；3）环境因素对胚胎发育影响的机制研究。分别在Molecular Cell、PNAS、Stem Cell Reports等杂志发表SCI收录论文50余篇。先后承担华中农业大学引进人才科研启动经费项目、国家重点研发项目“干细胞及转化研究”重点专项、国家自然基金面上项目和专项项目、国家转基因重大专项、国家科技支撑计划子项目、湖北省自然科学基金创新群体项目等多项科研项目。

教育经历

1997.09 - 2001.06，山东农业大学，畜牧专业毕业，农学学士
2001.09 - 2004.06，山东农业大学，动物遗传育种与繁殖专业毕业，农学硕士
2005.09 - 2008.06，中国科学院动物研究所，生理学专业毕业，理学博士
2008.08 - 2009.08，美国密苏里大学哥伦比亚分校，博士后

工作经历

2009.08-2013.03，美国国立卫生研究院环境健康科学研究所（NIH/NIEHS），访问学者（visiting fellow）
2013.03-2014.09，美国国立卫生研究院环境健康科学研究所（NIH/NIEHS），研究学者（research fellow）
2014.09-今，华中农业大学教授、博士生导师

研究领域

动物早期胚胎发育和体细胞重编程的机制胚胎干细胞的构建与自我更新机制研究环境因素对胚胎发育影响的机制基因敲除动物模型的构建与应用

科研成果

著作类
1.Wu DY, Zhang X, Miao YL*. Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer. Methods Mol Biol. 2019;2045:271-281.
论文类（#共一作者，*通讯作者）
1.Liu X#, Hao Y#, Li Z, Zhou J, Zhu H, Bu G, Liu Z, Hou X, Zhang X, Miao YL*. Maternal Cytokines CXCL12, VEGFA, and WNT5A Promote Porcine Oocyte Maturation via MAPK Activation and Canonical WNT Inhibition. Front Cell Dev Biol. 2020 Jul 7;8:578.
2.Zhang K#, Wu DY#, Zheng H#, Wang Y#, Sun QR, Liu X, Wang LY, Xiong WJ, Wang Q, Rhodes JDP, Xu K, Li L, Lin Z, Yu G, Xia W, Huang B, Du Z, Yao Y, Nasmyth KA, Klose RJ, Miao YL*, Xie W*. Analysis of Genome Architecture during SCNT Reveals a Role of Cohesin in Impeding Minor ZGA. Mol Cell. 2020 Jul 16;79(2):234-250.e9.
3.Zhou J#, Ji T#, He HN, Yin SY, Liu X, Zhang X, Miao YL*. Induction of autophagy promotes porcine parthenogenetic embryo development under low oxygen conditions. Reprod Fertil Dev. 2020 Apr;32(7):657-666.
4.Zhang JJ#, Liu X#, Chen L, Zhang S, Zhang X, Hao C*, Miao YL*. Advanced maternal age alters expression of maternal effect genes that are essential for human oocyte quality. Aging (Albany NY). 2020 Feb 25;12(4):3950-3961.
5.Chen L#, Zhang JJ#, Zhang X, Liu X, Zhao S, Huo LJ, Zhou J, Miao YL*. Melatonin protects against defects induced by malathion during porcine oocyte maturation. J Cell Physiol. 2020 Mar;235(3):2836-2846.
6.Zhou J#, Xue Z#, He HN, Liu X, Yin SY, Wu DY, Zhang X, Schatten H, Miao YL*. Resveratrol delays postovulatory aging of mouse oocytes through activating mitophagy. Aging (Albany NY) 2019 Dec 13;11(23):11504-11519.
7.Yin SY, Chen L, Wu DY, Wang T, Huo LJ, Zhao S, Zhou J, Zhang X*, Miao YL*. Tris(1,3-dichloro-2-propyl) Phosphate (TDCPP) disturbs mouse embryonic development by inducing apoptosis and abnormal DNA methylation. Environ Mol Mutagen. 2019 Dec;60(9):807-815.
8.Chen L#, Yin T#, Nie ZW, Wang T, Gao YY, Yin SY, Huo LJ, Zhang X, Yang J*, Miao YL*. Survivin regulates chromosome segregation by modulating the phosphorylation of Aurora B during porcine oocyte meiosis. Cell Cycle. 2018;17(21-22):2436-2446.
9.Zhou J, Li C, Yao W, Alsiddig MC, Huo L, Liu H*, Miao YL*. Hypoxia-inducible factor-1α-dependent autophagy plays a role in glycolysis switch in mouse granulosa cells. Biol Reprod. 2018 Aug 1;99(2):308-318.
10.Miao YL*, Gambini A, Zhang Y, Padilla-Banks E, Jefferson WN, Bernhardt ML, Huang W, Li L, Williams CJ*. Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse. Biol Reprod. 2018 Apr 1;98(4):449-464.
11.Liu X#, Nie ZW#, Gao YY, Chen L, Yin SY, Zhang X, Hao C*, Miao YL*. Sodium fluoride disturbs DNA methylation of NNAT and declines oocyte quality by impairing glucose transport in porcine oocytes. Environ Mol Mutagen. 2018 Apr;59(3):223-233.
12.Nie ZW, Chen L, Jin QS, Gao YY, Wang T, Zhang X, Miao YL*. Function and regulation mechanism of Chk1 during meiotic maturation in porcine oocytes. Cell Cycle. 2017;16(22):2220-2229.
13.Tao C#, Li J#, Zhang X, Chen B, Chi D, Zeng Y, Niu Y, Wang C, Cheng W, Wu W, Pan Z, Lian J, Liu H*, Miao YL*. Dynamic Reorganization of Nucleosome Positioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes. Stem Cell Reports. 2017 Aug 8;9(2):642-653.
14.Wang T, Gao YY, Chen L, Nie ZW, Cheng W, Liu1 X, Schatten H, Zhang X*, Miao YL*. Melatonin prevents postovulatory oocyte aging and promotes subsequent embryonic development in the pig. Aging 2017 Jun 26;9(6):1552-1564.
15.Zhang X, Liu X, Chen L, Wu DY, Nie ZW, Gao YY, Miao YL*. Caffeine delays oocyte aging and maintains the quality of aged oocytes safely in mouse. Oncotarget. 2017 Mar 28;8(13):20602-20611.
16.Gao YY, Chen L, Wang T, Nie ZW, Zhang X, Miao YL*. Oocyte aging-induced Neuronatin (NNAT) hypermethylation affects oocyte quality by impairing glucose transport in porcine. Sci Rep. 2016 Oct 26;6:36008.
17.Miao YL, Stein P, Jefferson WN, Padilla-Banks E, Williams CJ. Calcium influx-mediated signaling is required for complete mouse egg activation. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4169-74
18.Miao YL and Williams CJ. Calcium signaling in mammalian egg activation and embryo development: the influence of subcellular localization. Molecular Reproduction and Development. 2012 Nov;79(11):742-56.
19.Miao YL, Zhang X, Zhao JG, Zhao MT, Spate L, Murphy CN, Prather RS, Sun QY, Schatten H. Effects of griseofulvin on in vitro porcine oocyte maturation and embryo development. Environmental and Molecular Mutagenesis. 2012 Aug;53(7):561-6.
20.Chang G#, Miao YL#, Zhang Y, Liu S, Kou Z, Ding J, Chen DY, Sun QY, Gao S. Linking incomplete reprogramming to the improved pluripotency of murine embryonal carcinoma cell-derived pluripotent stem cells. PLoS One. 2010 Apr 26;5(4):e10320.
21.Miao YL, Kikuchi K, Sun QY, Schatten H. Oocyte aging: cellular and molecular changes, developmental potential, and reversal possibility. Human Reproduction Update. 2009 Sep-Oct;15(5):573-85.
22.Miao YL, Sun QY, Zhang X, Zhao JG, Zhao MT, Spate L, Prather RS, Schatten H. Centrosome abnormalities in porcine oocytes during aging. Environmental and Molecular Mutagenesis. 2009 Oct;50(8):666-71.
23.Miao YL, Shi LH, Lei Zl, Huang JC, Yang JW, Ouyang YC, Sun QY, Chen DY. Effects of caffeine on in vivo and in vitro oocyte maturation in mice. Theriogenology. 2007 Sep 1; 68(4):640-5.
24.Miao YL, Liu XY, Qiao TW, Miao DQ, Luo MJ, Tan JH. Cumulus cells accelerate aging of mouse oocytes. Biology of Reproduction. 2005 Nov; 73(5):1025-31.
25.Miao YL, Ma SF, Liu XY, Miao DQ, Chang ZL, Luo MJ, and Tan JH. Fate of the first polar bodies in mouse oocytes. Molecular Reproduction and Development. 2004 Sep; 69(1):66-76.

科研项目

1. 猪初始态（naive）多能干细胞系建立及多能性调控机制解析，国家重点研发项目“干细胞及转化研究”重点专项2016.01
2. Med13在卵母细胞减数分裂成熟、早期胚胎和生殖道发育中的功能研究，国家自然基金面上项目，2015.01
3. “十三五”国家转基因重大专项优质环保转基因猪新品种培育2016.01
4. “猪繁殖性状基因组学与种质创新”创新群体，湖北省自然基金，2018.01
5. 猪体细胞核移植胚胎发育过程中染色质开放与关键组蛋白修饰的动态变化及调控机制研究，国家自然基金面上项目，2020.01
6. 非洲猪瘟疫情下我国地方和新培育猪种种质资源新型保存方法的研究，国家自然基金专项项目，2020.01

主要奖励

1. 2011年获获得国家人口和计划生育委员会优秀科技成果奖一等奖（卵母细胞减数分裂机制及卵子质量控制）。